To determine whether tamoxifen could alter Twist1 protein stability, we treated HEK293 cells with inducible Twist1 expression and 168FARN cells with endogenous Twist1 expression with either cycloheximide or cycloheximide and tamoxifen, and then examined Twist1 protein degradation at different time points.
Tamoxifen (Nolvadex; AstraZeneca, Wilmington, DE) is a selective estrogen-receptor modulator (SERM) and is approved by the US Food and Drug Administration (FDA) for breast cancer risk reduction in both premenopausal and postmenopausal women.
If you want to look it up yourself, just go to Cancer Research's main page and type in Tamoxifen.
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The medium is then changed to MEM supplemented as described above, except that it contains 2% charcoal dextran-treated calf serum and various concentrations of Tamoxifen.
This suggests that the results of this study apply to healthy women in this age group, not just to those with comorbidities.
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When the cells were transfected with anti-miR-378a-3p, however, tamoxifen-mediated suppression of cell growth was impaired in MCF-7 cells ().
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